看完NovaSeq 5000/6000的技術參數表，有以下感觸：

1、測序策略越來越靈活

Flexible Performance

The NovaSeq 5000 and NovaSeq 6000 Systems offertremendous flexibility in sequencing options, supporting discovery across an extensive range of applications. Users can mix and match between the 4 flow cell types (S1, S2, S3, orS4*), run 1 or 2 flow cells at a time, and choose between multiple read lengths to easily adjust output for each sequencing run. The systems are compatible with Illumina library preparation kits, supporting a wide range of methods, from expression profiling to WGS and beyond (Table 3). Now users can run what they need, when they need it.

針對不同的測序場景，NovaSeq提供更多樣的選擇。從1~2個flow cell的自選，從flow cell類型 S1，S2，S3和S4（6000支持S1~S4,5000只支持S1~S2）。從測序讀長的PE50，PE100到PE150。總有一個組合適合你的。

2、儀器的後續升級擴展

Exclusively available on the NovaSeq 6000 System, the NovaSeq S3 and S4 flow cells* provide high throughput, while enabling cost effective WGS across a range of species and depths of coverage. Using the NovaSeq S3 orS4 flow cells makes performing WGS in house attractive and affordable for more labs. The NovaSeq 5000 System is fully upgradable to the NovaSeq 6000 System for future extensibility as the lab grows.*

相比6000 ，5000感覺存在感好低啊，不過如果樣品或者預算不高的話，可以先購買5000，隨著業務的增加，可以讓illumina升級，是機器升級不是換機子。這點illumina還是比較厚道，不過升級的費用不知多少。不知道有沒有測序公司集齊了 illumina的發布的所有測序機器（從最初的 GAI GAII家族，Nextseq500,NextSeq550家族，Miseq家族 Miniseq家族， Hiseq1000 Hiseq2000 Hiseq2500 Hiseq3000 Hiseq4000 Hiseq X家族）。

3、Streamlined Operation（更為自動化或者流水線的操作）

The NovaSeq 5000 and NovaSeq 6000 Systems increase lab efficiency through innovative user design features, workflow simplification, and reduced hands-on time. Advanced features include (Figure 3): l Load-and-go reagent cartridges that represent an 80%reduction in consumables compared to the HiSeq Series of Systems l RFID-encoded consumables enable automated reagent traceability and ensure compatibility of all sequencing reagents and flow cells l Automated flow cell loading and onboard cluster generation minimize hands-on time and variability due to manual processing

分別從 提升用戶交互界面（界面確實看起來清爽一些），簡化工作流程（比如樣品提取，建庫和測序），減少手工操作（類似於proton）提升效率。通過 Load-and-go的試劑放置盒（不知道怎麼翻譯好，下文會有補充）策略，比Hiseq的測序儀試劑消耗減少80%；通過RFID編碼技術，跟蹤試劑消耗品，確保試劑和flow cell的匹配；flow cell的自動載入和flow cell上的長簇 減少人工時間和由於人工操作的不一致性。後續會有更為詳細的說明。

3.1 Load-and-Go Reagents

The NovaSeq Series replaces dozens of tubes and bottles with 1
preconfigured sequencing by synthesis (SBS) reagent cartridge and 1
cluster generation reagent cartridge for each flow cell run. The
cartridges require no preparation other than thawing, eliminating the
need for user intervention, minimizing opportunities for errors, and
drastically reducing run setup time. In all, it takes only minutes to set up
a run on the NovaSeq 5000 or NovaSeq 6000 System.

還是簡化測序儀結構，每個run只需要 1個 synthesis (SBS) reagent cartridge，1個 cluster generation reagent cartridge。一個是邊合成邊測序的試劑裝載盒，一個是長簇的試劑裝載盒。不需要更多的人工操作，減少了測序啟動時間。

實驗過程的質控是非常重要的，這是每個實驗室的軟實力，特別是樣品多的時候，否則測出的都是不能用的數據，後續信息分析再怎麼做也是徒勞。這次illumina加強自動化的流程，提升試劑的利用率，客觀也是再降低成本（包括人工導致的測序質量下降甚至整個run的壞掉）。

The NovaSeq 5000 and NovaSeq 6000 Systems feature fully
automated onboard cluster generation, significantly reducing hands on
time. Prepared libraries are loaded directly into a sample tube that
sits in a preconfigured reagent cartridge, which is loaded directly onto
the system for fully automated cluster generation.

這部分還是文庫樣品的導入和簇的自動生成

The NovaSeq Series is part of a comprehensive sequencing and
informatics ecosystem that includes automated workflow
management with BaseSpace? Clarity Laboratory Information
Management System (LIMS). Data generated on a NovaSeq 5000 or
NovaSeq 6000 System can be streamed directly into BaseSpace
Sequence Hub forimmediate analysis and interpretation.

提升內在的樣品管理系統（這部分不是很懂，不說了），BaseSpase對於中國的用戶來說意義不大（需要再請教更懂的專家）。

4、Cutting-Edge Technology （不翻譯了）

The NovaSeq Series represents the most powerful, simple, scalable,
and reliable high-throughput Illumina sequencing platforms to date,
producing impeccable data quality. At the core of these systems is the
proven Illumina SBS chemistry. More than 90%of the worlds
sequencing data are generated by Illumina SBS chemistry.? This
proprietary reversible terminator–based method enables the massively
parallel sequencing of billions of DNA fragments, detecting single
bases as they are incorporated into growing DNA strands. The method
significantly reduces errors and missed calls associated with strings of
repeated nucleotides (homopolymers).

在測序鹼基質量方面，似乎這次沒有提升啊。

5、Ingenious Design

The NovaSeq Series unites the latest high-performance imaging with
the next generation of Illumina patterned flow cell technology to deliver
massive increases in throughput.

The superior optics of the NovaSeq Series offer high-resolution, high speed
scanning that represents a significant increase relative to the
HiSeq X
? System. This quicker scan speed contributes to making the
NovaSeq 5000 and NovaSeq 6000 Systems the highest throughput
Illumina sequencing platforms yet.

光學系統有提升，信號解析度和掃描速度都有提升。高的解析度可能分辨由於更高簇密度帶來的信號雜亂，更快的掃描速度減少測序時間（個人推斷，請專家指正）。

The NovaSeq Series leverages patterned flow cells, first deployed on
the HiSeq X System, with an improved, higher density design. Each
flow cell contains billions of nano wells at fixed locations, a design that
provides even cluster spacing and uniform feature size to deliver
extremely high cluster density. The redesigned NovaSeq flow cell
design further reduces the spacing between nano wells, significantly
increasing cluster density and data output. Combining this increase in
cluster density with the proprietary exclusion amplification clustering
method maximizes the number of nanowells occupied by DNA clusters
originating from a single DNA template. The result is an escalation in
data output.

這裡果然說了提升簇密度的方法，每個flow cell 可以容納更多的nano wells ，包括減少well之間的空隙。

以上內容選自 illumina.com/content/da

配圖來自：pbs.twimg.com/media/C1w


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